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shUFSP2#2 targets CDS: GCTGAAGACCTGCAAGTTATT) were obtained from Sigma-Aldrich Advanced Genomics ( and subsequently incorporated into the pLKO.1 lentiviral vector

Cancer 45 , 228247 (2009)

These details allow other researchers to replicate your methods

LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling